Mitochondria cytochrome oxidase I gene
PCR primers

C1-J-2195 MTD-10 TTG ATT TTT TGG TCA TCC AGA AGT
L2-N-3014 MTD-12 TCC AAT GCA CTA ATC TGC CAT ATT A

UBC Insect Mitochondrial DNA Primer Oligonucleotide Set, compiled by B. J. Crespi and C. Simon, with most taken from Simon et al., (1994)

PCR protocols
2µl of each template DNA
total reaction volume of 25 ul
0.15 µM dinucleotide triphosphates (dNTPs)
2.5 mM MgCl2,
0.75 units of Taq polymerase
0.6 uM each primer
PCR conditions
initial 2min at 95C (denaturation)
1 min at 95C (denaturation)
52C for 1 min (primer annealing)
1 min at 72C (amplification) for 30 cycles
5 min at 72C
PCR products are fractionated on 0.9% agarose gels, and bands were visualized by ethidium bromide staining and viewed with ultraviolet light

Source: Frohlich DR, Torres-Jerez I, Bedford ID, Markham PG, Brown JK. 1999. A phyogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Molecular Ecology: 1683-1691.